Dylight 550 spectra

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August undefined, 2024

WebCF® Dye Spectrum: CF®350 Dye. CF®350 is a blue fluorescent dye. Learn More. 300 400 500 600 700 800 900 Wavelength (nm) CF350. Technical Summary. ... 555, ATTO 550, Cy®3, DyLight® 549, Rhodamine. Features. Brighter than Cy®3 Highly water-soluble Validated in multicolor STORM super-resolution imaging. WebAlexa Fluor dyes are closely related to DyLight dyes because of structural similarities. Alexa Fluor® 488. Alexa Fluor 488 (Amax 493 nm, Emax 519 nm) has absorption and emission spectra comparable to Fluorescein (FITC), but are much brighter, far more photostable and less sensitive to pH changes between pH 4 and 10. Alexa Fluor 488 conjugates ...

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WebHiLyte FluorTM 555 550 566 5-TAMRA 541 568 Alexa Fluor®546 556 573 532 DyLight®550 562 576 Phycoerythrin (PE) 496,566 576 488 Tetramethyl Rhodamine … Web10 1040.06 0.139 mg of DyLight 550 NHS . Ester 150,000 MW 2 mg IgG × × = 13.9. μl of DyLight 550 NHS Ester 1 mg 100 μl 0.139 mg of DyLight 550 NHS Ester × = D. … coastlines of russia

Frequently asked questions - DyLight® 650 repla ... - Abcam

WebDyLight™ 650 is a red-emitting fluorophore that excited by the 640 nm laser and collected using a 670/30 nm bandpass filter. DyLight 650 has an excitation peak at 652 nm and an emission peak at 672 nm, and is spectrally similar to Alexa Fluor 647 and Cy5. ... Covering the entire visible light spectrum and several key near-infared and infrared ... WebDyLight™ 405 - conjugated secondary antibodies are excited maximally at about 400 nm and fluoresce with a peak at about 421 nm. They are very bright and photostable, but their optimal use requires instruments … WebThe DyLight® fluorophore spectra are shown below. Table: Spectral properties of DyLight® fluorochromes. In order to expand the spectra, please click on the spectrum … californium game

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Table of Fluorochromes - Salk Institute for Biological Studies

WebStore product protected from light at 4°C until opened. To extend the shelf-life of this product, add an equal volume of glycerol to make a final concentration of approximately 50% glycerol and store at -20°C. DyLight 550 Amax= 562 nm; Emax= 576 nm. Mole Dye/Mole Protein Ratio is lot-dependent. californium 251 half lifeWebJan 21, 2010 · Absorption and fluorescence emission spectra of DyLight 594 free and labeled on IgG are presented in Fig. 1.This dye can be conveniently excited from 530 to 610 nm.Many excitation sources like a green 532 nm, 543 nm and 568 nm lasers, as well as Rhodamine 6G dye laser operate in this spectral region.Most types of photodetectors are … coastline song download

"WebMar 6, 2024 · IHC-P. 1/50 - 1/500. DyLight ® 550 replaces DyLight ® 549. The DyLight ® 550 excitation is 562nm and the emission is 576nm. DyLight ® 550 provides the same orange-to-red fluorescence and photostability as the DyLight ® 549 dye. Please refer to the vial in order to check whether the product you received is DyLight ® 549 or DyLight ® 550. " - Dylight 550 spectra

Dylight 550 spectra

Fluorescent Dyes Science Lab Leica Microsystems

WebTable of Fluorochromes. This is a table of some characteristics of fluorochromes useful for flow cytometry or fluorescence microscopy. Within groups, roughly in order of excitation wavelength (families excepted). Peak excitation and emission wavelengths often vary depending on the environment in which the probe finds itself. WebJan 18, 2024 · Spectrum Viewer; SpectroFlo ® Services keyboard_arrow_up. Training Validation Shop; Applications keyboard_arrow_down. Research Areas keyboard_arrow_up. Immuno-oncology Infectious Diseases Inflammatory Diseases Workflows keyboard_arrow_up. Drug Discovery

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WebFAQs for Lightning-Link (R) Rapid DyLight 550 Kit (323-0005). (Showing 1 - 8 of 8 FAQ). Can I label a peptide/protein/antibody other than IgG? Yes. The Lightning-Link® technology works by targeting free amine groups on your target, meaning it can be used to label most biomolecules.As the protocols provided were optimized for labeling IgGs, we would … Web4. What is the excitation and emission of the DyLight® 550 fluorochrome? The excitation is 562nm and the emission is 576nm. As a comparison, the excitation of DyLight® 549 was also 562nm and the emission was …

WebIHC-P. 1/50 - 1/500. DyLight ® 550 replaces DyLight ® 549. The DyLight ® 550 excitation is 562nm and the emission is 576nm. DyLight ® 550 provides the same orange-to-red fluorescence and photostability as the DyLight ® 549 dye. Please refer to the vial in order to check whether the product you received is DyLight ® 549 or DyLight ® 550. WebTable 2. Properties of the Thermo Scientific DyLight Dyes. DyLight Dye A. max * ε† CF ‡ 350 . 405 . 488 . 550 . 594 . 633 . 650 . 680 . 755 . 800 * Excitation wavelength in nanometers − note that upon protein conjugation the absorption maximum shifts to the right of the spectra †Molar extinction coefficient (M-1. cm-1) at A. max ...

WebUnleash account specific instruments, save, collaborate, and export spectra viewer reports. WebAbout DyLight 550. DyLight™ 550 is a yellow-emitting fluorophore that can be excited by the 561 nm yellow-green laser and collected using a 582/15 nm bandpass filter. DyLight …

WebCatalog number: 62263. Thermo Scientific DyLight 550 Amine-Reactive Dye is an NHS ester-activated derivative of high-performance DyLight 550 used to fluorescently label …

WebJan 10, 2024 · TRITC is excited with light in the green spectrum with a maximum at 550 nm. Its emission maximum is lying at 573 nm. The bond to proteins (e.g. antibodies) is also based on a reactive isothiocyanate group. ... DyLight 405: 399 nm: 434 nm: DyLight 350: 332 nm: 435 nm: BFP (Blue Fluorescent Protein) 380 nm: 439 nm: Alexa 350: 343 nm: … californo ovensWebStorage: Upon receipt store DyLight NHS Esters at -20°C. Products are shipped with an ice pack. Store all dyes in the foil pouch with desiccant to protect from light and moisture. … californium 252 price per ounceWebThe excitation and emission spectra of the DyLight Fluor series cover much of the visible spectrum and extend into ... (488 nm) laser for DyLight 488, a green (526 nm) laser for … coastline speech therapyWebCatalog number: 62262. Thermo Scientific DyLight 550 Amine-Reactive Dye is an NHS ester-activated derivative of high-performance DyLight 550 used to fluorescently label … DyLight 550 provides vibrant orange-to-red fluorescence with better performance … TaqMan Real-Time PCR Assays. Antibodies. Oligos, Primers & Probes californo pia ovens reviewsWebSpectra Viewer. Dyes similar to ATTO 550 based on emission ... Stain CellTrace Yellow CF535ST CF540 CF550R CF568 CF568ST CMA-Chromomycin A3 Cy3 Cyanine 555 CytoTrace Red DiI dsRed dTomato DY-395XL DyLight 549 DyLight 550 Dyomics 547 eFluor 570 eFluor 585NC ExoBrite 560/585 EV Membrane Stain FVS570 FVS575V … californi wood burning fireplacesWebThe Thermo Scientific™ DyLight™ PEGylated Dyes display absorption maxima spectra ranging from 550nm to 783nm (Table 1). These reagents fluoresce over a broad pH range, are more intense than Alexa Fluor ... DyLight 550, CF555 : DyLight 650-4xPEG . 658 / 681 . 250,000 . 1425 : Alexa Fluor 647, Cy5, DyLight 650, CF647 . DyLight 680-4xPEG . … coastline specialty supplyWebStorage: Upon receipt store DyLight NHS Esters at -20°C. Products are shipped with an ice pack. Store all dyes in the foil pouch with desiccant to protect from light and moisture. Introduction . The Thermo Scientific DyLight Amine-Reactive Dyes have absorption spectra ranging from 350nm to 770nm (Table 1) and are packaged in a convenient ... coastlines on the mediterranean sea