Tth polymerase antibody
WebAug 5, 2024 · Background: As a heat-resistant polymerase, Thermus thermophilus (Tth) DNA polymerase can be widely used in Polymerase Chain Reaction (PCR). However, its non-specific amplification phenomenon is serious, which greatly limits development. Objective: In this study, we prepared Tth monoclonal antibodies against Tth DNA polymerase and … WebPolymerase By similarity. BLAST Add. Sequence: Expand table. Sequence similarities. Belongs to the DNA polymerase type-A family. Curated. Family and domain databases. CDD. cd08637 DNA_pol_A_pol_I_C 1 hit; cd09898 H3TH_53EXO 1 hit; cd09859 PIN_53EXO 1 hit; Gene3D. 3.30.70.370 1 hit;
Tth polymerase antibody
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WebTth Polymerase. Tth Polymerase is a thermostable DNA polymerase originally isolated from Thermus thermophilus. It has a 5’-3’ polymerase activity, a 3’-5’ exonuclease activity, and, in the presence of Mn 2+, an additional reverse transcriptase activity. It is active at 74°C and can withstand up to 95°C. WebHot Start technology, using anti-Tth DNA polymerase antibodies, allows for high specificity and reproducible intercalation assay. This product is a 2 × Master Mix for "1-step real-time PCR" using a thermostable DNA polymerase derived from Thermus thermophilus (Tth) HB8).
WebAbstract. The ability of thermostable DNA polymerases to mediate template-dependent DNA synthesis in the presence of phenol has been examined as monitored by amplification of a specific Borrelia burgdorferi rRNA sequence. Tth DNA polymerase displayed the unique property of maintaining both DNA- and RNA-dependent DNA polymerase activities in the ... WebAug 8, 1997 · These aptamers have been shown to efficiently inhibit the polymerase activity of Taq pol and are useful in enhancing the amplification efficiency of low copy number …
WebAnti-Taq/Tth Hotstart Antibody available. ... Tth DNA Polymerase TTH-3R 10x Buffer for Tth 1mL 10x Reaction Buffer for Tth. CAT# TTH Range EC# 2.7.7.7 E N Z Y M E S M: 100bp … WebExample 1. Detection of influenza virus RNA. Influenza virus RNAs were detected using the Hot Start TTx (RNA) Kit and Tth DNA Polymerase-based real-time PCR reagent. When 1 µL diluted, purified RNA was included in the 20µL reaction solution, the Hot Start TTx (RNA) Kit was more sensitive than the Tth DNA Polymerase-based reagent. Example 2.
WebTth DNA Polymerase activity is resistant to prolonged incubations at high temperatures (+95°C), and can therefore be used for PCR amplification. In the presence of manganese …
WebTth Polymerase 5 U/µl is a thermostable DNA polymerase with intrinsic reverse transcription activity, but no RNAse H activity. The enzyme has a size of 94 kDA and was isolated from … north face triclimate youthWebNational Center for Biotechnology Information how to save quickbooks fileWebThis product is a 2 × Master Mix for "1-step real-time PCR" using a thermostable DNA polymerase derived from Thermus thermophilus (Tth) HB8 (1). Tth DNA polymerase … north face triclimate womens saleWebMar 1, 2008 · Antibodies were also produced by injecting hybridomas into the peritoneal cavity of 8-week-old Balb/c mice. Ascetic fluids with high antibody titers ... Rapid and sensitive reverse transcriptase-polymerase chain reaction based detection and differential diagnosis of fish pathogenic rhabdoviruses in organ samples and cultured ... north face trinity sleeping bagWebTth DNA Polymerase is isolated from the thermophilic eubacterium Thermus thermophilus HB8, and is purified to be free of nonspecific DNases and RNases. The enzyme is a highly … north face trivert hoodie menWebJan 2, 2024 · The capacity of r Tth polymerase to detect DNA in the presence of different concentrations of Na + ions, Ca 2+ ions, Fe 3+ ions, EDTA, heparin, bile salts, IgG, lactoferrin, hemoglobin, and myoglobin has been reported [ 11 – 13, 21 ]. There is also evidence that RNA detection by r Tth polymerase is more resistant to the inhibitors present in ... north face trucker hat saleWebApr 3, 2011 · Taq Polymerase. The Taq polymerase enzymes utilized in these TaqMan assays (either Taq or Tth) possess a 5′ to 3′ exonuclease activity, and as a result, ... The antibody-mediated inhibition of Taq polymerase allows for room temperature assembly of the PCR reaction mixture. north face trinity jacket